Анотация: During a five-year period (2000 to 2004) 74,342 pigs were tested by the intradermal tuberculin test in Croatia. Of them, 248 (0.33) pigs were positive and 91 (0.12) were found to be suspicious in 7 out of the 13 farms included in the study. Gross pathological changes characteristic of tuberculosis were observed in tuberculin-positive and/or suspicious swine. Mycobacterium was isolated from the lymph nodes of 183 out of 234 swine (78.2). For better epidemiological understanding, isolates were typed by conventional methods, PCR and hybridisation. The results show that most of the isolates belonged to the Mycobacterium avium complex (175 isolates, 95.7). Other isolates belonged to M. fortuitum (6 isolates, 3.3), M. chelonae (1 isolate, 0.5), and M. peregrinum (1 isolate, 0.5). Isolated strains of the M. avium complex were identified as M. a. avium (37 isolates, 21.1) and M. a. hominissuis (138 isolates, 78.9).