Анотация: The genetic diversity of Sclerotinia sclerotiorum populations recovered from canola was assessed using rep-PCR genomic fingerprinting. By using four rep-PCR primers, 1927 polymorphic bands out of a total of 2003 (96.2 percentage) were generated in 38 isolates of S. sclerotiorum. At the species level, Nei's gene diversity (h) was 0.233 and Shannon's index of diversity (I) was 0.376. Genetic diversity based on percentage of polymorphic bands ranged from 59.5 percentage to 75.21 percentage, demonstrated high level of genetic diversity. The cluster analysis based on UPGMA and Jaccardфs coefficient showed that most isolates from the same regions were grouped in the same cluster or a close cluster. The Nie's genetic identity illustrated that populations from Hashem Abad and Ali Abad were genetically close, while the population from Kalaleh was found to be the most diverse from the others. The variability found within closely related isolates of S. sclerotiorum demonstrate the effectiveness of rep-PCR marker in identifying genetic diversity among S. sclerotiorum isolates