Анотация: The genetic variability of three native chicken populations (Dashtiari, Khazak and Zabol) derived from Sistan and Baluchistan province in Iran was evaluated with 10 microsatellites markers (MCW5, MCW16, MCW18, MCW34, MCW39, ADL225, ADL262, ADL185, ADL136, ADL210). The mean heterozygosity was 0.77, 0.79 and 0.52 for Khazak, Dashtiari and Zabol chickens, respectively. The highest PIC was 0.81 in ADL225 and ADL136 at Dashtiari and Khazak chickens. The results of the heterozygosity were consistent with polymorphism information content (PIC). The genetic information in the current investigation will be used in conservation and improvement program of three native chickens