Анотация: Increased resistance of Staphylococcus spp. is a growing problem in veterinary medicine. This study was performed to examine antimicrobial susceptibility of Staphylococcus pseudintermedius and other bacteria isolated from canine skin and ear infections over a 7 year period: 2000-2006. Eighty-six dogs which met predetermined inclusion criteria were examined. Bacterial isolates were examined for susceptibility to four commonly used antibiotics: cephalothin (first generation cephalosporin), amoxicillin-clavulanate, potentiated sulfonamide and enrofloxacin. The degree of susceptibility and the percentage of resistant strains of the bacteria to the antibiotics were compared between two periods: 2000-2002 and 2003-2006. Comparison between sample sites and association between susceptibility and signalment were examined. Resistance of S. pseudintermedius and Proteus spp. to cephalosporins and amoxicillin-clavulanate increased over the years and it was statistically significant for S. pseudintermedius (P=0.018). Susceptibility of S. pseudintermedius to cephalosporins and amoxicillin-clavulanate decreased over the years (P= 0.009). Susceptibility of Proteus spp. to amoxicillin-clavulanate and fluoroquinolones decreased as well (P=0.037, P=0.055 respectively). More skin than ear isolates of S. pseudintermedius and Proteus were resistant to cephalosporins and amoxicillinclavulanate. More ear isolates of Proteus were resistant to potentiated sulfonamides (P= 0.039). All isolates of Proteus from mix breed dogs, but not from German shepherds were resistant to cephalosporins (P=0.032). This study showed an increased resistance and decreased susceptibility of two common bacteria found in dogs` skin and ear infections. These findings should encourage clinicians to submit samples for culture and susceptibility before commencing antibacterial therapy, so as to provide a more accurate therapeutic decision and avoid unnecessary antibiotic use