Анотация: The isolation of pure and high molecular weight genomic DNA is a pre-requisite for many molecular biology applications including the polymerase chain reaction. Various protocols are available for the isolation of DNA from plant materials. Two protocols: cetyl trimethyl ammonium bromide protocol and benzyl chloride protocol, have been optimized for isolation of genomic DNA in essential oil of Cymbopogon sp. as available standard protocols do not produce high quality PCR amplifiable DNA. Spectrophotometric and electroporetic analyses indicated that the isolated DNA according to both methods was highly pure and could be amplified by using RAPD primers