Andonova, M.; Urumova, V.; Petkova, B.; Slavov, E.; Dzhelebov, P.; Chaprazov, Ts.; Roydev, R.; Borissov, I.
Haematological and biochemical parameters characterising the progression of experimental Pseudomonas aeruginosa skin infection in dogs
: BULGARIAN JOURNAL OF VETERINARY MEDICINE
: 2014 .
: 17, 1
: p. 32-41
: TUN
:  1190

: dogs






 

 

 

 

 

 

 

 
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: The aim of the study was to investigate the changes in some rapid, indicative clinical laboratory parameters - white blood cells (WBC), leukogram, erythrocyte sedimentation rate (ESR), total protein (TP), albumin (A), globulins (G), albumin/globulin ratio/style> ;

ТsPU `? @A?  `? @? &? 0 ? &@? &?  ? ? v?  0#  D?  ? 0&?  ?  ? ?  ? @?  ) `L? ? ? x?  + `L?  ? ? =x+ywp?   ?  ?  X[?   ?  ?  B? >? s`QЋ? ? ? Т? X? ? /? Qs*Qsa6X*? ? @?  5? C? ? c:B`@B? ? 0 ? ? `? ` ?  ?  ?  ? '?  ?  ? H? `? <? @? sh? 5Xs ? u? ? ? ? ? ? ? ? ?  ? ? ? ? ? ? ؈ ? X[? ? ? ? ,IL? s? ? ?  ?  (A/G) during experimental Pseudomonas aeruginosa skin infection in dogs and to determine their ability to provide information for evaluating such type of infection. Suspension of P.aeruginosa (1108 cfu/mL) was inoculated at a dose 0.3 mL/kg body weight, in five clinically healthy, dogs, 2-5 years old, weighing 24.3 +- 1.8 kg. The control group (n=5) was injected with the same dose of normal saline. The blood samples were taken in the following dynamics: before infection (0 h) and on 4th, 24th, 48th, 72nd hour and on 7th, 10th and 14th day after infection. The data presented clearly suggest that ESR was the most sensitive haematological parameter increasing significantly 4 hours after infection (P<0.01), with values remaining high (P<0.001) till the end of experimental period (day 14). WBC increased significantly on hour 72 (P<0.001). The changes in the leukogram demonstrated increase in band neutrophils on hour 48 and 72 (P<0.01), which is indicative for left shift. Eosinopenia was found on hour 24 and 72 (P<0.01). Analysis of biochemical parameters demonstrated that the period within 24th to 72nd hour was crucial in progress of P. aeruginosa skin infection. This statement is supported by the significant decrease of albumin concentration (P<0.001), decrease in A/G ratio and hyperglobulinaemia (P<0.001) within this period. These alterations in protein profile did not affect total protein concentration, which remained unchanged during the whole experimental period. The matched analysis of both haematological and biochemical parameters is more accurate and indicative for the progression of bacterial skin infections in dogs. Albumin and globulins concentrations and A/G ratio are sensitive, consistent and reliable parameters, which can be useful for evaluating skin P. aeruginosa infection in dogs