Анотация: Brucellosis is a worldwide zoonotic disease that still constitutes a major public health problem in Syria. In the current study, Brucella has been isolated from cow milk samples and typed by both molecular and conventional techniques. Over a 6-year period (2002-2007) 2372 milk samples were collected from Syrian cow herds. The milk ring test (MRT) was performed where 57% of samples were found to be positive. Milk samples were cultured on the Brucella selective medium and incubated for 24Ц48 h. One-quarter of milk samples were contaminated with Brucella, which is considered as a relatively high rate of contamination. In addition, biochemical properties of isolates have been identified. All isolated Brucella strains were positive for catalase, oxidase and urea reaction, did not need CO2 to grow and rarely produced H2S, could grow in the presence of fuchsin and thionin. The agglutination test was negative with anti-R and positive with anti-M and anti-A sera; so it was determined as B. melitensis strain, biovar 3. A specific PCR assay targeting the bcsp31 gene and the specific integration of IS711 elements within the genome of Brucella species has been used. Molecular typing results were compatible with conventional methods