Анотация: Mycotoxins are secondary metabolites produced by fungi which can affect a variety of feedstuffs. These compounds elicit toxicological effects which represent risk for both humans and animals. This study aimed to investigate the presence of mycotoxins Zearalenone ( ZEA ), Ochratoxin A ( OTA ), Deoxinivalenol ( DON ), Aflatoxin, Fumonisin as natural contaminants of maize, wheat, barley and sunflower. The samples were analyzed by Enzyme-linked immunosorbent assay - ELISA. A total number of 59 samples had been investigated. The content of DON for barley was estimated in the range between 0.544 and 2.29 mg/kg. The contamination with aflatoxin was between 2 and 14.49 g/kg. The highest concentration was estimated in one sample of sunflower. More favorable substrate for Fumonisins accumulation was maize. The occurrence of ZEA varied from 51.65 g/kg to 126.54 g/kg in wheat and 48.67-79.64 g/kg in barley. OTA contamination was between 4.86- 7.32 g/kg in wheat and 4.46-11.70 g/kg in barley respectively. These results suggest a high percentage of ZEA and OTA, especially in wheat and barley