Description: Dorema ammoniacum D. Don. (Apiaceae) which is native to Iran is classified as a vulnerable medicinal species. The aim of this study was to enhance the germination of Dorema ammoniacum seeds, which have a low percentage under normal laboratory conditions. Different pretreatments were carried out to obtain efficacy in breaking dormancy and speeding germination, using of scarification, cold stratification, Washing and chilling treatments, gibberellic acid (GA3) and N6-benzyladenine (BA) treatments. The rate and percentage of seed germination varied with duration and medium of stratification. The highest germination percentage was obtained in the moist sand cold stratification for 45 days (68.00 +- 1.7). Both germination rate and percentage of Dorema ammoniacum increased by increasing the number of exposure days at 4 C. However, when the chilling temperature was decreased from 4 to -5 and -10 C, a negative response was monitored for both germination rate and percentage. Addition of GA did not promote germination. Regardless of applied treatment to seeds, germination rate was positively correlated with germination percentage. Therefore, fast germination was associated with high germination percentage. For the first time, these results indicated that Dorema ammoniacum seeds which have deep morphophysiological dormancy need a chilling treatment for reliable germination