Description: A crossbreed bitch, one year old, was brought to the Obstetrics and Reproduction Clinic at the Faculty of Veterinary Medicine, University of Zagreb for elective castration. From the history, the bitch had last been on heat three months before and did not mate. Clinical examination was performed before surgery, the health status and condition of the animal were good and at the owner`s request no preoperative diagnostic methods were performed. Laparoscopic ovariohisterectomy of the enlarged uterus was performed under general inhalated anesthesia. The pathohistological finding was segmental endometrial hyperplasia of the uterus. According to this case report laparoscopic ovariohysterectomy is safe and effective in bitches with small to moderate enlargement of the uterus