Description: This research describes the detection of six honeybee viruses in samples of clinically affected Carniolan gray bee collected between 2007 and 2009 on the territory of Slovenia. Using one-step reverse transcription-PCR (RT-PCR), 60 bee samples originated from 45 apiaries were screened for the presence of six honeybee viruses. Samples were found positive for acute bee paralysis virus (ABPV = 40 percentage), black queen cell virus (BQCV = 83,3 percentage), chronic bee paralysis virus (CBPV = 18,3 percentage), deformed wing virus (DWV = 70 percentage), Kashmir bee virus (KBV = 1,7 percentage) and sacbrood bee virus (SBV = 8,3 percentage). Mortality and paralysis were often evident in the apiaries and could be connected with ABPV and/or CBPV infections. Both viruses were detected in clinically affected apiaries with high bee mortality and with paralysis symptoms showing flightless bees, trembling and crawling at the hive entrance. The severity of clinical manifestation with high bee losses were associated with higher number of viruses detected in the samples. Among virus positive samples, 27 percentage of them were infected with one virus, 30 percentage with two viruses, 25 percentage with three viruses and 15 percentage of samples contained four viruses simultaneously. The results of this study provide data about the detection of several bee viruses in affected bee colonies and viral infections in Carniolan bee have to be investigated by further research