Анотация: Currently, due to their beneficial effects, there is interest in adding prebiotics to food products. This study investigated the effect of the addition of inulin (1 percentage and 2 percentage) on microbial and physico-chemical properties of probiotic low fat yogurt manufactured with Lactobacillus acidophilus. Six experimental preparations of yogurt were produced. Homogenized, standardized and pasteurized low fat milk were divided into six portions. Four portions were fortified with 1 percentage and 2 percentage inulin and two portions were used without inulin. All of the preparations were heated up to 85рC and fermented at 42рC until a pH of 4.6 was reached. Titratable acidity and pH were determined during the incubation period of the samples and a storage time up to 14 days. Syneresis, color, sensory evaluation and bacterial counts were determined during the storage time. The results showed that inulin did not significantly affect the titratable acidity and pH of the yogurts after 4 h of fermentation at 40рC. There were no significant differences between pH, titratable acidity, syneresis, color and sensory evaluation of all treatments on days 1, 7 and 14 of storage. The counts of L. acidophilus and L. delbrueckii ssp. bulgaricus declined over time, but the addition of inulin to the milk increased the viability of these bacteria during the storage of synbiotic yogurt. In conclusion, inulin can be used to manufacture low fat synbiotic yogurt with additional nutritional benefits without affecting the physico-chemical properties of yogurt