Анотация: Detection and isolation of pathogenic Yersinia enterocolitica strains from foods of animal origin is difficult and inefficient in many cases. During the last decade, many classical microbiological and immunochemical methods are successfully complemented with molecular based techniques. In the current study, an optimized protocol for Real-Time PCR and an efficient system for detection of ail gene in Yersinia enterocolitica is presented. The selected primer pair I?1/MP2 with the MPFR as a positive control and TaqManо probe were successfully applied for detection and quantification of Yersinia enterocolitica WA 314 strain of bio/serotype 1b/O:8. The established protocol could be implemented for detection and quantitation of pathogenic Yersinia enterocolitica strains in clinical samples like meat, milk, faeces, etc