Анотация: Cereus peruvianus callus have a lot of alkaloids compounds and medicine extracted, which is important in medicine, medical and agricultural industries. The experiments were performed using Complete Randomized Design (CRD) and results were analyzed using one way ANOVA. MS medium supplemented with different concentrations of (2,4-D), kinetin and NAA were used. The study was carried out on Increasing callus weight in two different seasons of Cereus peruvianus var. monstrosus and Cereus peruvianus var. tortuosus. Results showed the callus induction of Cereus peruvianus var. tortuosus in MS supplemented with 4 mg/L of 2, 4-D in summer and 4 mg/L of NAA in spring was highest, and in MS medium supplemented with (6mg/l) of kinetin in spring and summer was the lowest for Cereus peruvianus var monstrosus. There were significant differences between two varieties of callus weight. Also there were significant differences between media treatment in summer and spring