Анотация: Swine influenza disease is not included in the National Prophylaxis Programme and the reports about spreading of the virus among pigs are not sufficient. The infection in pigs is usually asymptomatic, but the possible expression of clinical symptoms could result in lower productivity and economic losses. In earlier investigations concerning the spreading of swine influenza viruses (SIV) in Bulgaria, a viral circulation among the swine population was proved. For this reason and because of the high probability of emergence of new strains of unknown infectious potential, it is important to keep current diagnostic readiness in the National Reference Laboratory of Avian Influenza and Newcastle Disease Virus. The goal of the present study was to compare different protocols and combinations of kits and primer/probe sets in order to establish а rRT-PCR protocol for fast SIV diagnosis. To achieve this goal several commercial kits for rRT-PCR detection of SIV were used. After evaluation of the indicators we found that the confidence interval for the Combo M gene protocol in combination with the three used extraction kits ranged between 22.65% and 77.4%, contrasting to the combinations of used in-house amplification protocols with QIAamp Viral RNA Mini Kit (QIAGEN, Germany) and NucleoSpin RNA Virus (Macherey-Nage, Germany) extraction kits - between 67.05% and 100%. By rRT-PCR, we were able to detect RNA from all swine viruses used in this study. Our results confirmed that the correct detection of swine influenza virus is possible using a protocol different from that of the EU Reference Laboratory