Description: The aim of this study was to compare the lateral and medial digital bones of the left and right thoracic and pelvic limbs of water buffaloes by direct anatomical measurements. This study was conducted on 240 digital bones of ten native Khuzestan buffalo bulls, aged 2 to 3 years. No significant differences were found between corresponding measurements of digital bones of the right and left limbs nor between the total lengths of the digital bones of the lateral and medial digits within the same limbs (p>0.05). The symmetric digital bones may have contributed to distribute an equal weight on the foot during standing or walking and might explain why biomechanical lesions of the feet in water buffaloes are much less frequent than in cattle. Moreover, data acquired in the present study might be useful for researchers working on osteoarchaeological excavations as reference values for evaluation and identification of digital bones of water buffaloes among artiodactyl species