Description: The isolation of Pasteurella multocida from several red-necked wallabies (Macropus rufogriseus) with purulent to necrotizing gingivitis, mandibular osteomyelitis, and conjunctivitis in a private zoological park prompted a cross-sectional prevalence study in two zoological collections. The study demonstrated a high prevalence of P. multocida carriage in clinically healthy red-necked wallabies. In one collection (n = 7), P. multocida was isolated from the gingival mucosa of two animals (28.6 percentage) and the conjunctiva of one animal (14.3 percentage). In another collection (n = 29), P. multocida was isolated from the buccal mucosa in two animals (6.9 percentage) and the pharyngeal mucosa of eight animals (27.6 percentage). Multilocus sequence typing showed that sequence types varied among, but were identical within, collections