Description: A survey was conducted to determine the incidence of Johne's disease (JD) in goat flocks at Qassim region, central Saudi Arabia. Cases of JD were recorded in eighteen out of 90 goat herds (20) (n = 2610). All infected goats were adult of more than 3 years of age. Twelve herds had single cases, while the other 6 herds presented more than one affected animal (range 2-5). The total number of confirmed JD cases was 29 which amounted to 1.11 of the total number of goats in the original 90 herds. Nine goats at extremis were humanely killed and autopsied. The lesions of the disease were confined to the intestine and the liver, with one animal showing remote pancreatic involvement. Gross lesions were enlarged mesenteric lymph nodes and thickened corrugated intestinal mucosa. The pancreas was fluffy white. Histopathological examination showed infiltration of the intestinal mucosa and mesenteric lymph nodes with epithelioid cells. The liver had two types of granulomas: a lepromatous type (which was more dominant), and a tuberculoid type that was characterized by presence of Langhans giant cells. There was a massive destruction of the exocrine pancreas in one goat. Clumps of short slender acid-fast bacilli were isolated in pure culture on Herrold's egg yolk agar medium from faeces and various lesions including the liver granuloma. The organism was identified as Mycobacterium avium subsp. paratuberculosis on the basis of its slow growth and strict mycobactin dependence.