Description: The invertase activity and carbohydrate spectrum of organic acacia and polyfloral honeys produced in the South Stara Planina region (Republic of Bulgaria) were investigated. After one year of storage at a temperature up to 25 С, a mild reduction in invertase activity (3.9-4.79 percentage) has occurred in polyfloral honey. The activity of this enzyme decreased considerably in acacia honey (15.16 percentage). The average content of carbohydrates of acacia honey after one-year storage was: fructose - 42.76+-1.24 g/100 g, glucose - 27.5+-1.11 g/100 g, fructose/glucose ratio - 1.55+-0.05, sucrose - 0.60+-0.63 g/100 g, turanose - 1.18+-0.21 g/100 g, maltose - 0.54+-0.36 g/100 g, trehalose - 0.28+-0.04 g/100 g. The respective carbohydrates in polyfloral honey were fructose (41.16+-0.81 g/100 g), glucose (27.11+-0.85 g/100 g), fructose/glucose ratio (1.51+-0.07), sucrose (0.14+-0.20 g/100 g), turanose (1.17+-0.26 g/100 g), maltose (0.90+-0.69 g/100 g), trehalose (0.65+-0.19 g/100 g). Neither melibiose nor melezitose were established in both types of honey