Description: In vitro study was carried out to determine the antibacterial activity of root extracts of Berberis lycium by using various solvents for extraction. The methanolic root extract showed higher inhibitory effect as compared to the other solvent extracts, against the mixed culture of human pathogenic bacteria viz., Pseudomonas sp., Escherchia coli, Streptococcus sp. and Staphylococcus sp. However, methanolic extract when used separately were less effective compared to Gentamycin and Amoxicillin showing inhibition zone of 10, 11 and 12 against Staphylococcus, Pseudomonas sp. and E. coli respectively