Description: Listeria monocytogenes is a Gram positive, facultative intracellular pathogen with the capacity to cause food poisoning outbreaks as well as severe illness in vulnerable human population groups. In this paper the faecal carriage rate of Listeria monocytogenes in domestic dogs in Isfahan city, determined from 92 faecal samples by bacteriological methods. Listeria monocytogenes was isolated from only one of the samples examined (1.08%). The serotype of isolated bacteria was 1/2b. It was concluded that stray dogs could be a reservoir of the organism as well as a source of human listeriosis in Iran. This is the first report on the isolation of Listeria monocytogenes from domestic dogs in Isfahan city